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Table 1 Saccharomyces cerevisiae strains

From: The Irr1/Scc3 protein implicated in chromosome segregation in Saccharomyces cerevisiae has a dual nuclear-cytoplasmic localization

Strain Genotype Source
IRR1 MAT a ade2—1 his3-11,15 leu2-3112 trp1-1 ura3-1 can1-100 Rothstein collection (Columbia University, New York, USA)
2281a MAT a/α {ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1::IRR1-GFP/kanMX4 spc42::SPC42-mCherry/natMX6} D. Ivanov, Inst. Mol. Cell Biology, Singapore [25]
2239a MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1::IRR1-GFP/kanMX4 spc42::SPC42-mCherry/natMX6 D. Ivanov
PJ69-4α MAT α trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ [33]
Irr1-GFP/Imi1-RFP MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1::IRR1-GFP/kanMX4
[pCM189-IMI1-RFP, URA3, CEN] 		This study, derivative of 2239
Irr1-GFP/RFP MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1::IRR1-GFP/kanMX4
[pCM189-RFP, URA3, CEN] 		This study, derivative of 2239
GFP/Imi1-RFP MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 imi1::natMX6 [pUG34-GFP, HIS3, CEN] [pCM189-IMI1-RFP, URA3, CEN] This study, derivative of 2239
MNY7 MAT a trp1 his3 leu2 ura3 crm1::kanR [pDC-CRM1, LEU2, CEN] [34]
MNY8 MAT a trp1 his3 leu2 ura3 crm1::kanR [pDC-crm1-T539C, LEU2, CEN] [34]
MNY7-IRR1-GFP MAT a trp1 his3 leu2 ura3 crm1::kanR IRR1::irr1-GFP/kanMX4 spc42::SPC42-mCherry/natMX6
[pDC-CRM1, LEU2, CEN] 		This study, derivative of MNY7
MNY8-IRR1-GFP MAT a trp1 his3 leu2 ura3 crm1::kanR IRR1::irr1-GFP/kanMX4 spc42::SPC42-mCherry/natMX6
[pDC- crm1-T539C, LEU2, CEN] 		This study, derivative of MNY8
irr1(V248E)-GFP MAT a ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 irr1:: kanMX4
[pUG35-irr1(V248E)-GFP, URA3, CEN] 		This study, derivative of IRR1
irr1(F986A)-GFP MAT a ade2-1 his3-11,15 leu2-3112 trp1-1 ura3-1 can1-100 irr1:: kanMX4
[pUG35-irr1(F986A)-GFP, URA3, CEN] 		This study, derivative of IRR1
Irr1(V248E, F986A)-GFP MAT a ade2-1 his3-11,15 leu2-3112 trp1-1 ura3-1 can1-100 irr1:: kanMX4
[pUG35-irr1(V248E, F986A)-GFP, URA3, CEN] 		This study, derivative of IRR1
  1. aIn these strains the IRR1 gene was originally named SCC3 by Dr. Ivanov
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Cell Division

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