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Plasmid
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Description
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Source:
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|---|
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pACTII
|
P
ADH1
-Gal4-AD-MCS- T
ADH1
Amp
R
LEU2 2μ
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Clontech #638822
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|
pGBKT7
|
P
ADH1
-Gal4-DNA-BD-MCS-T
T7 & ADH1
Kan
R
TRP1 2 μ
|
Clontech #630489
|
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pGBKT7-C-Irr1
|
P
ADH1
-Gal4-DNA-BD-Irr1Δ1-474-T
T7 & ADH1
Kan
R
TRP1 2μ
|
This study
|
|
pCM189
|
P
tetO
-MCS-T
CYC1
Amp
R
URA3 CEN
|
[37]
|
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pCM189-P
tetO
-IMI1-RFP
|
P
tetO
-IMI1-RFP-T
CYC1
Amp
R
URA3 CEN
|
[30]
|
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pCM189-P
tetO
-RFP
|
P
tetO
-RFP-T
CYC1
Amp
R
URA3 CEN
|
This study
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pUG23
|
P
MET25
-MCS-GFP-T
CYC1
Amp
R
HIS3 CEN
|
U. Güldener and J. H. Hegemann, Heinrich-Heine-Universität, Düsseldorf, Germany
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pUG34
|
P
MET25
-GFP-MCS-T
CYC1
Amp
R
HIS3 CEN
|
